Atg16L1 as a Novel Biomarker and Autophagy Gene for Diabetic Retinopathy

被引:9
|
作者
Gao, Xinxiao [1 ,2 ]
Du, Yunhui [3 ]
Lau, Wayne Bond [4 ]
Li, Yu [3 ]
Zhu, Siquan [2 ]
Ma, Xin-Liang [1 ,4 ]
机构
[1] Capital Med Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100069, Peoples R China
[2] Capital Med Univ, Beijing Anzhen Hosp, Dept Ophthalmol, Beijing 100029, Peoples R China
[3] Capital Med Univ, Beijing Inst Heart Lung & Blood Vessel Dis, Beijing Anzhen Hosp, Beijing 100029, Peoples R China
[4] Thomas Jefferson Univ, Dept Emergency Med, 1025 Walnut St,Coll Bldg,Suite 808, Philadelphia, PA 19107 USA
基金
北京市自然科学基金; 中国博士后科学基金;
关键词
D O I
10.1155/2021/5398645
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. Accumulating evidence suggests the critical role of autophagy in the pathogenesis of diabetic retinopathy (DR). In the current study, we aim to identify autophagy genes involved in DR via microarray analyses. Methods. Gene microarrays were performed to identify differentially expressed lncRNAs/mRNAs between normal and DR retinas. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of lncRNA-coexpressed mRNAs were used to determine the related pathological pathways and biological modules. Real-time polymerase chain reactions (PCR) were conducted to validate the microarray analyses. Results. A total of 2474 significantly dysregulated lncRNAs and 959 differentially expressed mRNAs were identified in the retina of DR. Based upon Signalnet analysis, Bcl2, Gabarapl2, Atg4c, and Atg16L1 participated the process of cell death in DR. Moreover, real-time PCR revealed significant upregulation of Atg16L1. Conclusion. This study indicated the importance and potential role of Atg16L1, one of the autophagy genes, as a biomarker in DR development and progression.
引用
收藏
页数:11
相关论文
共 50 条
  • [1] Atg16I1: a novel biomarker and autophagy gene for diabetic retinopathy identified by microarray analysis
    Gao, Xinxiao
    Du, Yunhui
    Lau, Wayne Bond
    Zhu, Siquan
    Ma, Xin-Liang
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2020, 61 (07)
  • [2] The Autophagy Gene Atg16L1 is Necessary for Endometrial Decidualization
    Oestreich, Arin K.
    Chadchan, Sangappa B.
    Popli, Pooja
    Medvedeva, Alexandra
    Rowen, Marina N.
    Stephens, Claire S.
    Xu, Ran
    Lydon, John P.
    Demayo, Francesco J.
    Jungheim, Emily S.
    Moley, Kelle H.
    Kommagani, Ramakrishna
    ENDOCRINOLOGY, 2020, 161 (01)
  • [3] Determination of autophagy gene ATG16L1 polymorphism in human colorectal cancer
    Nicoli, Elena-Raluca
    Dumitrescu, Theodor
    Uscatu, Constantin-Daniel
    Popescu, Florin-Dan
    Streata, Ioana
    Sosoi, Simona Serban
    Ivanov, Petar
    Dumitrescu, Alexandra
    Barbalan, Alexandru
    Lungulescu, Dan
    Petrescu, Florin
    Schenker, Michael
    Verdes, Doina
    Saftoiu, Adrian
    ROMANIAN JOURNAL OF MORPHOLOGY AND EMBRYOLOGY, 2014, 55 (01): : 57 - 62
  • [4] Differential Involvement of Atg16L1 in Crohn Disease and Canonical Autophagy ANALYSIS OF THE ORGANIZATION OF THE Atg16L1 COMPLEX IN FIBROBLASTS
    Fujita, Naonobu
    Saitoh, Tatsuya
    Kageyama, Shun
    Akira, Shizuo
    Noda, Takeshi
    Yoshimori, Tamotsu
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (47) : 32602 - 32609
  • [5] A Deficiency in the Autophagy Gene Atg16L1 Enhances Resistance to Enteric Bacterial Infection
    Marchiando, Amanda M.
    Ramanan, Deepshika
    Ding, Yi
    Gomez, Luis E.
    Hubbard-Lucey, Vanessa M.
    Maurer, Katie
    Wang, Caihong
    Ziel, Joshua W.
    van Rooijen, Nico
    Nunez, Gabriel
    Finlay, B. Brett
    Mysorekar, Indira U.
    Cadwell, Ken
    CELL HOST & MICROBE, 2013, 14 (02) : 216 - 224
  • [6] The multifaceted functions of ATG16L1 in autophagy and related processes
    Gammoh, Noor
    JOURNAL OF CELL SCIENCE, 2020, 133 (20)
  • [7] A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells
    Ken Cadwell
    John Y. Liu
    Sarah L. Brown
    Hiroyuki Miyoshi
    Joy Loh
    Jochen K. Lennerz
    Chieko Kishi
    Wumesh Kc
    Javier A. Carrero
    Steven Hunt
    Christian D. Stone
    Elizabeth M. Brunt
    Ramnik J. Xavier
    Barry P. Sleckman
    Ellen Li
    Noboru Mizushima
    Thaddeus S. Stappenbeck
    Herbert W. Virgin IV
    Nature, 2008, 456 : 259 - 263
  • [8] ATG16L1 functions in cell homeostasis beyond autophagy
    Hamaoui, Daniel
    Subtil, Agathe
    FEBS JOURNAL, 2022, 289 (07) : 1779 - 1800
  • [9] A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells
    Cadwell, Ken
    Liu, John Y.
    Brown, Sarah L.
    Miyoshi, Hiroyuki
    Loh, Joy
    Lennerz, Jochen K.
    Kishi, Chieko
    Kc, Wumesh
    Carrero, Javier A.
    Hunt, Steven
    Stone, Christian D.
    Brunt, Elizabeth M.
    Xavier, Ramnik J.
    Sleckman, Barry P.
    Li, Ellen
    Mizushima, Noboru
    Stappenbeck, Thaddeus S.
    Virgin, Herbert W.
    NATURE, 2008, 456 (7219) : 259 - U62
  • [10] The role of ATG16L1 in bacterial-induced autophagy
    Hussey, S.
    Yuan, L.
    Travassos, L.
    Philpott, D.
    Jones, N.
    INFLAMMATORY BOWEL DISEASES, 2009, 15 (12) : S54 - S54