A potentiometric and spectroscopic study on the interaction between human immunoglobulin g and sodium perfluorooctanoate in aqueous solution

被引：0
|
作者：
Messina, Paula V.
Prieto, Gerardo
Sabin, Juan
Blanco, Elena
Varela, Carla
Dodero, Veronica
Ruso, Juan M.
Sarmiento, Felix [1 ]
机构：
[1] Univ Santiago de Compostela, Fac Phys, Biophys & Interfaces Grp, E-15782 Santiago De Compostela, Spain
[2] Univ Santiago de Compostela, Fac Biol, Dept Biochem & Mol Biol, E-15782 Santiago De Compostela, Spain
[3] Univ Santiago de Compostela, Dept Organ Chem, E-15782 Santiago De Compostela, Spain
来源：
MACROMOLECULAR SYMPOSIA | 2007年 / 251卷
关键词：
human immunoglobulin G; monolayers; sodium perfluorooctanoate; unfolding;
D O I：
10.1002/masy.200750514
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
The interaction between human immunoglobulin G (IgG) and sodium perfluorooctanate (SPFO) has been characterized by a combination of UV-vis and UV-CD spectroscopies and ion selective electrodes. The study determined that there were true specific unions between SPFO and IgG. The conformational changes at the bulk solution, induced by SPFO, were well characterized by Uv-vis and UV-CD spectroscopies. Chemical and thermal unfolding were analysed and the thermodynamic parameters were determined.
引用
收藏
页码：103 / 111
页数：9
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