Complete sequences of IncHI1 plasmids carrying blaCTX-M-1 and qnrS1 in equine Escherichia coli provide new insights into plasmid evolution

被引:44
|
作者
Dolejska, Monika [1 ,2 ]
Villa, Laura [3 ]
Minoia, Marco [4 ]
Guardabassi, Luca [4 ]
Carattoli, Alessandra [3 ]
机构
[1] Univ Vet & Pharmaceut Sci Brno, Dept Biol & Wildlife Dis, Fac Vet Hyg & Ecol, Brno 61242, Czech Republic
[2] Univ Vet & Pharmaceut Sci Brno, CEITEC VFU, Brno 61242, Czech Republic
[3] Ist Super Sanita, Dept Infect Parasit & Immune Mediated Dis, I-00161 Rome, Italy
[4] Univ Copenhagen, Dept Vet Dis Biol, DK-1870 Frederiksberg C, Denmark
关键词
CTX-M-1; IncHI1; IncX1; horses; fructooligosaccharides; QUINOLONE RESISTANCE; GENOMIC ISLAND; ENABLES; GENES; TYPHI;
D O I
10.1093/jac/dku172
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: To determine the structure of two multidrug-resistant IncHI1 plasmids carrying bla(CTX-M-1) in Escherichia coli isolates disseminated in an equine clinic in the Czech Republic. Methods: A complete nucleotide sequencing of 239 kb IncHI1 (pEQ1) and 287 kb IncHI1/X1 (pEQ2) plasmids was performed using the 454-Genome Sequencer FLX system. The sequences were compared using bioinformatic tools with other sequenced IncHI1 plasmids. Results: A comparative analysis of pEQ1 and pEQ2 identified high nucleotide identity with the IncHI1 type 2 plasmids. A novel 24 kb module containing an operon involved in short-chain fructooligosaccharide uptake and metabolism was found in the pEQ backbones. The role of the pEQ plasmids in the metabolism of short-chain fructooligosaccharides was demonstrated by studying the growth of E. coli cells in the presence of these sugars. The module containing the blaCTX-M-1 gene was formed by a truncated macrolide resistance cluster and flanked by IS26 as previously observed in IncI1 and IncN plasmids. The IncHI1 plasmid changed size and gained the quinolone resistance gene qnrS1 as a result of IS26-mediated fusion with an IncX1 plasmid. Conclusions: Our data highlight the structure and evolution of IncHI1 from equine E. coli. A plasmid-mediated sugar metabolic element could play a key role in strain fitness, contributing to the successful dissemination and maintenance of these plasmids in the intestinal microflora of horses.
引用
收藏
页码:2388 / 2393
页数:6
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