GC/MS-Based Metabolomics Profiling Approach and Determination of Ameliorative Effect of Chiliadenus Montanus Extract towards CCl4 Induced Hepatotoxicity in Albinol Rats

The current study aimed at evaluating the GC/MS profiling of Chiliadenus montanus leaves extract which consists of 48 compounds, nine of them are major and represented 52.55 % of the total peak areas. The ameliorative effect towards the CCl4-induced hepatotoxicity in male Wistar rats and endogenous antioxidants content in the liver were analyzed. CCl4 diluted 1:9 (v/v) in olive oil was injected intraperitoneally followed by oral administration of methanolic plant extract (200 mg/kg body weight). The CCl4-treated rats showed a significant decline in the studied hematological parameters, the serum levels of high-density lipoprotein (HDL), albumin (A) as well as the hepatic levels of glutathione (GSH) and activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). This was accompanied by a significant elevation in the levels of total lipids (TL), triglycerides (TG), total cholesterol (TC), low-density lipoproteins (LDL), globulin (G), total bilirubin (TBil) and the activities of alanine and aspartate aminotransferase (ALAT and ASAT) and alkaline phosphatase (ALP) as well as the hepatic levels of malondialdehyde (MDA). In contrast, the administration of methanol extract, notably improved all the studied parameters. This study showed that CCl4 administration to Wistar rats, at a high dose level, could induce a hepatic injury in addition to certain hematologic and metabolic alterations. The work was extended to examine tissue histopathology. In conclusion, C. montanus leaves methanol extract, resulted in an attractive candidate for ameliorating of hepatotoxicity induced by CCl4 through scavenging free radicals, improved liver functions, and normalizing the liver histopathological architecture. Further studies are required in order to identify the molecules responsible of the pharmacological activities. Yet, the treatment with methanol extract could ameliorate these alterations via their antioxidative effect.