Improvement of amperometric glucose biosensor by the immobilization of FcCD inclusive complex and carbon nanotube

被引：22

作者：

Zheng, Longzhen ^{[1
]}

Li, Junhua ^{[1
]}

Xu, Jingpeng ^{[2
]}

Xiong, Leyan ^{[1
]}

Zheng, Dan ^{[1
]}

Liu, Qiang ^{[1
]}

Liu, Wen ^{[1
]}

Li, Yindi ^{[1
]}

Yang, Shaoming ^{[1
]}

Xia, Jian ^{[1
]}

机构：

[1] E China Jiao Tong Univ, Dept Chem & Chem Engn, Nanchang 330013, Jiangxi, Peoples R China

[2] Jiangxi Inst Anal & Testing, Nanchang 330029, Jiangxi, Peoples R China

来源：

ANALYST | 2010年 / 135卷 / 06期

基金：

中国国家自然科学基金;

关键词：

ELECTRON-TRANSFER MEDIATORS; HORSERADISH-PEROXIDASE; REDOX MEDIATORS; ELECTROCHEMICAL PROPERTIES; OXIDASE; SURFACE; GEL;

D O I：

10.1039/b925505b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel glucose biosensor was constructed by using ferrocene-carbonyl-beta-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter. FcCD inclusive complex and glucose oxidase (GOx) were covalently bonded to CNTs by poly-L-lysine (PLL) to fabricate a glucose biosensor. The electrocatalytic oxidation of glucose at the biosensor occurred at low potential below 200 mV, avoiding the interference of the main interfering substances in real samples containing a 5-times higher concentration of L-cysteine, ascorbic acid, and uric acid. The biosensor showed fast response for glucose. A broad linear range of glucose concentration from 1.0 x 10(-5) to 2.90 x 10(-3) M was obtained and the detection limit was 2.2 x 10(-6) M.

引用

页码：1339 / 1344

页数：6

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