Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster

被引：1
|
作者：
Kojima, Atsushi [1 ,2 ,3 ]
Osawa, Nanako [1 ,2 ]
Oba, Mami [1 ]
Katayama, Yukie [1 ]
Omatsu, Tsutomu [1 ,2 ]
Mizutani, Tetsuya [1 ,2 ]
机构：
[1] Tokyo Univ Agr & Technol, Ctr Infect Dis Epidemiol & Prevent Res, 3-5-8 Saiwai Cho, Fuchu, Tokyo 1830054, Japan
[2] Tokyo Univ Agr & Technol, Grad Sch Agr, Cooperat Div Vet Sci, 3-5-8 Saiwai Cho, Fuchu, Tokyo 1830054, Japan
[3] Little Bird & Small Anim Hosp Little Bird, Setagaya Ku, 1-46-16-202 Gotokuji, Tokyo 1540021, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2022年 / 84卷 / 02期
关键词：
26S rDNA D1; D2; genotyping; intergenic space 1; internal transcribed spacer; Macrorhabdus ornithogaster; SEQUENCE-ANALYSIS; DIVERSITY; CANARIES;
D O I：
10.1292/jvms.21-0576
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Macrorhabdus ornithogaster (MO) is an infectious fungus that causes gastric damage in birds. In this study, we established nested and seminested polymerase chain reaction (PCR) methods that specifically amplify the domain D1/D2 region (D1/D2) of 26S ribosomal DNA (rDNA), internal transcribed spacer (ITS) of rDNA, and intergenic spacer (IGS) 1 region from avian feces. Phylogenetic analysis of MO collected from Japanese pet birds showed little genetic variation; analysis based on these regions did not distinguish between host species order, differences in MO shape, or host gastrointestinal symptoms. These regions were found to be unsuitable for molecular epidemiological studies of MO and further investigation into other genetic regions is required.
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页码：244 / 250
页数：7
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