Structure of an acidic polysaccharide from the agar-decomposing marine bacterium Pseudoalteromonas atlantica strain IAM 14165 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid

被引：14
|
作者：
Perepelov, AV
Shashkov, AS
Torgov, VI
Nazarenko, EL
Gorshkova, RP
Ivanova, EP
Gorshkova, NM
Widmalm, G [1 ]
机构：
[1] Univ Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden
[2] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia
[3] Russian Acad Sci, Pacific Inst Bioorgan Chem, Far E Branch, Vladivostok 690022, Russia
来源：
CARBOHYDRATE RESEARCH | 2005年 / 340卷 / 01期
基金：
俄罗斯基础研究基金会;
关键词：
Pseudoalteromonas atlantica; polysaccharides; structure; NMR spectroscopy; marine bacteria; 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid; pseudaminic acid;
D O I：
10.1016/j.carres.2004.11.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structure of an acidic polysaccharide from Pseudoalteromonas atlantica strain 14165 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac) has been elucidated. The polysaccharide was studied by H-1 and C-13 NMR spectroscopy, including 21) experiments, along with sugar and methylation analyses. After a selective hydrolysis a modified polysaccharide devoid of its side chain could be isolated. It was found that the polysaccharide has pentasaccharide repeating units with following structure: P-Psep5Ac7Ac-(2 --> 6) \ -->3)-alpha-D-Galp-(1 --> 6)-alpha-D-GlepNAc-(1 --> 4)-alpha-D-GalpA-(1 --> 3)-beta-D-QuipNAc-(1 --> (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码：69 / 74
页数：6
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