Quantitative and dynamic measurements of biological fresh samples with X-ray phase contrast tomography

被引：19

作者：

Hoshino, Masato ^{[1
]}

Uesugi, Kentaro ^{[1
]}

Tsukube, Takuro ^{[2
]}

Yagi, Naoto ^{[1
]}

机构：

[1] Japan Synchrotron Radiat Res Inst, Res & Utilizat Div, Sayo, Hyogo 6795198, Japan

[2] Japanese Red Cross Kobe Hosp, Chuo Ku, Kobe, Hyogo 6510073, Japan

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2014年 / 21卷

关键词：

X-ray phase contrast tomography; quantitative imaging; three-dimensional densitometry; dynamic imaging; MICRO COMPUTED-TOMOGRAPHY; SYNCHROTRON-RADIATION; GRATING INTERFEROMETER; TALBOT INTERFEROMETRY; STRAIN FIELDS; RESOLUTION; SPECIMENS;

D O I：

10.1107/S1600577514018128

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

X-ray phase contrast tomography using a Talbot grating interferometer was applied to biological fresh samples which were not fixed by any fixatives. To achieve a high-throughput measurement for the fresh samples the X-ray phase contrast tomography measurement procedure was improved. The three-dimensional structure of a fresh mouse fetus was clearly depicted as a mass density map using X-ray phase contrast tomography. The mouse fetus measured in the fresh state was then fixed by formalin and measured in the fixed state. The influence of the formalin fixation on soft tissue was quantitatively evaluated by comparing the fresh and fixed samples. X-ray phase contrast tomography was also applied to the dynamic measurement of a biological fresh sample. Morphological changes of a ring-shaped fresh pig aorta were measured tomographically under different degrees of stretching.

引用

页码：1347 / 1357

页数：11

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