A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE

被引：19

作者：

Sugimoto, Shinya ^{[1
]}

Higashi, Chihana ^{[1
]}

Saruwatari, Kozue ^{[1
]}

Nakayama, Jiro ^{[1
]}

Sonomoto, Kenji ^{[1
]}

机构：

[1] Kyushu Univ, Fac Agr, Grad Sch, Dept B&B,Div MS&T,Lab MT,Higashi Ku, Fukuoka 8128581, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 16期

基金：

日本学术振兴会;

关键词：

molecular chaperone; DnaK; DnaJ; GrpE; gram-negative and -positive bacteria; cooperation;

D O I：

10.1016/j.febslet.2007.05.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe importance of the characteristic segment in ATPase domain of DnaK chaperone which is present in all gram-negative bacteria but is absent in all gram-positive bacteria. lit vitro studies, ATPase activity, luciferase-refolding activity, and surface plasmon resonance analyses, demonstrated that a segment-deletion mutant DnaK Delta 74-96 became defective in the cooperation with the co-chaperones DnaJ and GrpE. In addition, in vivo complementation assay showed that expression of DnaK Delta 74-96 could not rescue the viability of Escherichia coli AdnaK mutant at 43 degrees C. Consequently, we suggest evolutionary significance for this DnaK ATPase domain segment in gram-negative bacteria towards the DnaK chaperone system. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2993 / 2999

页数：7

共 2 条

[1] THE ATP HYDROLYSIS-DEPENDENT REACTION CYCLE OF THE ESCHERICHIA-COLI HSP70 SYSTEM - DNAK, DNAJ, AND GRPE
SZABO, A
LANGER, T
SCHRODER, H
FLANAGAN, J
BUKAU, B
HARTL, FU
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (22) : 10345 - 10349
[2] Production of active manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release
Alfi, Almasul
Zhu, Bo
Damnjanovic, Jasmina
Kojima, Takaaki
Iwasaki, Yugo
Nakano, Hideo
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2019, 128 (03) : 290 - 295

← 1 →