Silicon-rhodamine isothiocyanate for fluorescent labelling

被引:4
|
作者
Nasufovic, Veselin [1 ]
Then, Patrick [2 ]
Droge, Fabian [3 ]
Duong, Michael [1 ]
Kaether, Christoph [4 ]
Dietzek, Benjamin [2 ,3 ]
Heintzmann, Rainer [2 ,3 ]
Arndt, Hans-Dieter [1 ]
机构
[1] Friedrich Schiller Univ, Inst Organ Chem & Macromol Chem, Humboldtstr 10, D-07743 Jena, Germany
[2] Leibniz Inst Photon Technol Leibniz IPHT, Albert Einstein Str 9, D-07745 Jena, Germany
[3] Friedrich Schiller Univ, Inst Phys Chem, Helmholtzweg 4, D-07743 Jena, Germany
[4] Leibniz Inst Aging, Fritz Lipmann Inst FLI, Beutenbergstr 11, D-07745 Jena, Germany
关键词
SI-RHODAMINE; PROBES; FLUOROPHORE; ANTIBODY; NANOPARTICLES; MICROSCOPY; PLATFORM; DYES;
D O I
10.1039/d0ob02016h
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
An efficient synthesis for silicon-rhodamines was developed, enabling the preparation and evaluation of silicon-rhodamine isothiocyanate (SITC) as a novel tool for facile fluorescent labeling. Ease of use in conjugation to amino groups, high stability and excellent photophysical properties are demonstrated. SITC-actin was found to be neutral to F-actin polymerization induction and well suited for high resolution fluorescence microscopy.
引用
收藏
页码:574 / 578
页数:5
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