Lysinibacter cavernae gen. nov., sp nov., a new member of the family Microbacteriaceae isolated from a karst cave

A Gram-stain-positive, aerobic, straight or slightly bent rod-shaped, non-motile, non-spore-forming bacterium, designated strain CC5-806(T), was isolated from a soil sample collected from a wild karst cave in the Wulong region, Chongqing, PR China and examined using a polyphasic approach to clarify its taxonomic position. This bacterium did not produce substrate mycelium or aerial hyphae, and no diffusible pigments were observed on the media tested. Strain CC5-806(T) grew optimally without NaCl at 20 degrees C and at pH 7.0. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain CC5-806(T) belonged to the family Microbacteriaceae and showed the highest levels of 165 rRNA gene sequence similarities with Frigoribacterium endophyticum EGI 6500707(T) (97.56 %), Frigoribacterium faeni 8011 (97.53 %) and Glaciihabitans tibetensis MP203(T) (97.42 %). Phylogenetic trees revealed that strain CC5-806(T) did not show a clear affiliation to any genus within the family Microbacteriaceae. The DNA G+C content of strain CC5-806(T) was 62.6 mol%. The cell-wall peptidoglycan contained L-lysine as a diagnostic diamino acid. The predominant menaquinones were MK-11, MK-10 and MK-9. Phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid, four unidentified phospholipids and other polar lipids were detected in the polar lipid extracts. The major fatty acids were anteiso-C-15 :0, iso-C-16:0 and iso-C-14 :0. On the basis of the phylogenetic analysis, and phenotypic and chemotaxonomic characteristics, strain CC5-806(T) was distinguishable from phylogenetically related genera in the family Microbacteriaceae. It represents a novel species of a novel genus, for which the name Lysinibacter cavemae gen. nov., sp. nov. is proposed. The type strain is CC5-806(T) (=DSM 27960(T)=CGMCC 1.14983(T)).