Suppose that X is a Banach space and C is an injective operator in B(X),the space of all bounded linear operators on X. In this note, a two-parameter C-semigroup ( regularized semigroup) of operators is introduced, and some of its properties are discussed. As an application we show that the existence and uniqueness of solution of the 2-abstract Cauchy problem (partial derivative/(partial derivative t(i)))u(t(1),t(2)) = H(i)u (t(1),t(2)), i = 1, 2, t(i) > 0, u (0, 0) = x, x is an element of C(D(H(1))boolean AND D(H(2))) is closely related to the two-parameter C-semigroups of operators. Copyright (C) 2009 Mohammad Janfada.