Rapid method for the determination of the substitution pattern of O-methylated 1,4-glucans by high-pH anion-exchange chromatography with pulsed amperometric detection

A rapid method has been developed for the determination of the substitution pattern of methyl-starches, -amyloses, -celluloses and -cyclodextrins in the anhydro glucose unit. All eight constituents possible for this type of copolymers could be separated by high-pH anion-exchange chromatography with pulsed amperometric detection (PAD). Peaks were assigned by comparison with synthesized standard compounds. For quantitative evaluation the relative response factors of the O-methyl-glucose derivatives were determined.