Phaseolus vulgaris L. somatic embryos induction in liquid medium

被引：3
|
作者：
Svetleva, D
Nikolaev, P
Veltcheva, M
Petkova, S
Atanassov, A
机构：
[1] Higher Inst Agr, Plovdiv, Bulgaria
[2] Inst Genet Engn, Kostinbrod 2, Bulgaria
来源：
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 1999年 / 13卷 / 02期
关键词：
D O I：
10.1080/13102818.1999.10819029
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Bulgarian common bean (Phaseolus vulgaris L.) cultivars: the standard - Dobrudjanski 7 and the mutant - Plovdiv 11M were used as the donor genotypes. Direct somatic embryos have been induced from different explants (roots, hypocotyls, primary leaves and epycotyls or leaf petioles) in liquid media (modified B-5), containing different concentrations of 2.4-D. Amount and the quality of induced embryos depends on the genotype, the concentration of 2.4-D and the type and age of the explants. Conversion and germination of bean embryo development are crucial and have to be optimized.
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页码：9 / 13
页数：5
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