A draft genome for Spatholobus suberectus

被引:29
|
作者
Qin, Shuangshuang [1 ,2 ]
Wu, Lingqing [3 ]
Wei, Kunhua [2 ]
Liang, Ying [2 ]
Song, Zhijun [2 ]
Zhou, Xiaolei [2 ]
Wang, Shuo [2 ]
Li, Mingjie [1 ]
Wu, Qinghua [2 ]
Zhang, Kaijian [3 ]
Hui, Yuanyuan [3 ]
Wang, Shuying [3 ]
Miao, Jianhua [2 ]
Zhang, Zhongyi [1 ,4 ]
机构
[1] Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Fujian, Peoples R China
[2] Guangxi Bot Garden Med Plants, Guangxi Key Lab Med Resources Protect & Genet Imp, Nanning 530023, Peoples R China
[3] Novogene Bioinformat Inst, Beijing 100083, Peoples R China
[4] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Genet Breeding & Comprehens Utilizat Crop, Fuzhou 350002, Fujian, Peoples R China
基金
中国国家自然科学基金;
关键词
RNA; SEQUENCE; ANNOTATION; GENE; CLASSIFICATION; IDENTIFICATION; CONSTITUENTS; PREDICTION; PROVIDES; DATABASE;
D O I
10.1038/s41597-019-0110-x
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Spatholobus suberectus Dunn (S. suberectus), which belongs to the Leguminosae, is an important medicinal plant in China. Owing to its long growth cycle and increased use in human medicine, wild resources of S. suberectus have decreased rapidly and may be on the verge of extinction. De novo assembly of the whole S. suberectus genome provides us a critical potential resource towards biosynthesis of the main bioactive components and seed development regulation mechanism of this plant. Utilizing several sequencing technologies such as Illumina HiSeq X Ten, single-molecule realtime sequencing, 10x Genomics, as well as new assembly techniques such as FALCON and chromatin interaction mapping (Hi-C), we assembled a chromosome-scale genome about 798 Mb in size. In total, 748 Mb (93.73%) of the contig sequences were anchored onto nine chromosomes with the longest scaffold being 103.57 Mb. Further annotation analyses predicted 31,634 protein-coding genes, of which 93.9% have been functionally annotated. All data generated in this study is available in public databases.
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页数:9
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