Development of a class-specific monoclonal antibody-based ELISA for aflatoxins in peanut

被引：85
|
作者：
Li, Peiwu [1 ]
Zhang, Qi [1 ]
Zhang, Wen [1 ]
Zhang, Jinyang [1 ]
Chen, Xiaomei [1 ]
Jiang, Jun [1 ]
Xie, Lihua [1 ]
Zhang, Daohong [1 ]
机构：
[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Dept Qual Standard & Food Safety, Wuhan 430062, Peoples R China
来源：
FOOD CHEMISTRY | 2009年 / 115卷 / 01期
关键词：
Aflatoxins; Class-specific; Monoclonal antibody; Enzyme-linked immunosorbent assay (ELISA); FLUORESCENCE DETECTION; AFFINITY; IMMUNOASSAY; PRODUCTS; MILK; FOOD; B-1;
D O I：
10.1016/j.foodchem.2008.11.052
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Three class-specific monoclonal antibodies against aflatoxins were screened by a designed strategy in which aflatoxin G(2) was used as competitor in the screening ELISA system. With a high cross-reactivity (65%) to aflatoxin G(2), antibody 10C9 had the most similar sensitivity for five aflatoxins (AFB(1), AFB(2), AFG(1), AFG(2) and AFM(1)), whose I-50 values were in a range of 2.1-3.2 ng ml (1). So, antibody 10C9 was selected to develop an ELISA for determination of aflatoxin B-1, B-2, G(1), G(2) and total of them in peanut samples. And spiked recoveries were from 87.5% to 102.0%. The results indicate that the ELISA developed can accurately determine total aflatoxins in samples of peanuts after the simple and rapid extraction procedure. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：313 / 317
页数：5
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