Identifying novel autoantibody signatures in ovarian cancer using high-density protein microarrays

被引：25
|
作者：
Gunawardana, C. Geeth [1 ,2 ]
Memari, Nader [1 ,2 ]
Diamandis, Eleftherios P. [1 ,2 ,3 ]
机构：
[1] Mt Sinai Hosp, Dept Pathol & Lab Med, Toronto, ON M5T 3L9, Canada
[2] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada
[3] Univ Hlth Network, Dept Clin Biochem, Toronto, ON, Canada
来源：
CLINICAL BIOCHEMISTRY | 2009年 / 42卷 / 4-5期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Tumour-associated antigens; Protein microarray; Ovarian cancer; GENE;
D O I：
10.1016/j.clinbiochem.2008.11.008
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: To identify autoantibody signatures in ovarian cancer using protein microarray technology. Design and methods: Protein microarrays were screened using non-malignant peritoneal fluid (n=30) and ascites fluid pooled from ovarian cancer patients (n=30). Results: Fifteen potential tumour-associated antigens were discovered. AASDHPPT showed the strongest signal-to-noise ratio. Conclusions: Protein microarrays are suitable for autoantibody discovery in ovarian cancer but the signatures are of low frequency. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：426 / 429
页数：4
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