Background Reference intervals (RIs) of various blood biochemical parameters are used in assessing the clinical health and nutritional status of animals. Blood biochemical analyte RIs are not currently available in Shami goats. Objectives This study aimed to determine the RIs of selected blood biochemical analytes in Shami goats. Methods Blood from 120 animals (females N = 70; males N = 50) was used to determine the RIs of total protein (TP), albumin (ALB), globulin, fibrinogen (FIB), urea, creatinine (CRT), AST, ALT, ALP, GGT, glucose (GLU), calcium (Ca), sodium (Na), potassium (K), and chloride (CL) using commercially available kits. Results The RIs for selected blood biochemical analytes in Shami goats were: TP (34.9-83.5 g/L), ALB (22.3-55.1 g/L), globulin (9.9-50 g/L), FIB (2.19-5.45 g/L), urea (0.55-9.5 mmol/L), CRT (11.4-221 mu mol/L), aspartate aminotransferase (7.9-299 U/L), ALT (2.3-49 U/L), ALP (7.7-950 U/L), GGT (4.5-185 U/L), GLU (1.3-6.8 mmol/L), Ca (1.65-2.6 mmol/L), Na (120-180 mmol/L), K (3.7-6.3 mmol/L), CL (91.9- 125 mmol/L), and the albumin:globulin ratio (0.45-3.9). Conclusions The RIs of the selected blood biochemical analytes in Shami goats were determined in this study. These results can be used in the clinical diagnosis of diseases in Shami goats and to evaluate their general health and nutritional status.