Detecting the Infection of the Cabernet Sauvignon Variety of Clonal Origin by Grape Viruses

The high-yielding Cabernet Sauvignon grape variety is susceptible to viral diseases, which may influence the agrobiological and taste characteristics of its quality. The objectives of this study are to identify a Cabernet Sauvignon variety of clonal origin from the south of Ukraine, detect the infection of plants of this variety with harmful grape viruses incorporated into the certification system of planting materials, determine the causative agent of viral diseases by biomolecular methods, and establish the nucleotide sequence of the 2C(CP) envelope protein gene of detected grape viruses. As a result of phytosanitary survey, some Cabernet Sauvignon grape bushes of clonal origin with symptoms of grapevine fanleaf virus (CFLV) and grapevine leaf roll-associated virus (GLRaV) have been revealed. The results of grape virus identification by the real-time reverse-transcription polymerase chain reaction (RT-PCR) have demonstrated the presence of grapevine fanleaf virus in grape plants with infection symptoms. As a result of sequencing, it has been established that the nucleotide sequence of an isolate from the Cabernet Sauvignon variety is very close to the samples from regions geographically distant from Ukraine, first of all, the United States, Iran, and France. Based on microsatellite analysis, it has been proven that specifically the Cabernet Sauvignon variety of clonal origin is infected with grapevine fanleaf virus. The obtained 2C(CP) envelope protein gene sequence has been deposited with the international GenBank database with no. MN072356.1.