Molecular and biological properties of tomato necrotic stunt virus and development of a sensitive real-time RT-PCR assay

被引：11

作者：

Li, Rugang ^{[1
]}

Fei, Zhangjun ^{[2
]}

Ling, Kai-Shu ^{[1
]}

机构：

[1] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA

[2] Cornell Univ, Boyce Thompson Inst Plant Res, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA

来源：

ARCHIVES OF VIROLOGY | 2014年 / 159卷 / 02期

基金：

美国食品与农业研究所;

关键词：

SMALL RNAS; DISCOVERY; SEQUENCE;

D O I：

10.1007/s00705-013-1830-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The newly identified tomato necrotic stunt virus (ToNStV), with a genome of 10,057 nucleotides, had 60 % nucleotide sequence and 70 % coat protein amino acid sequence identity to other potyviruses. Analysis of a second ToNStV isolate revealed two single nucleotide substitutions. ToNStV induced stunting and chlorotic to necrotic leaf symptoms on tomato plants. In host-range assays, ToNStV systemically infected many species in the family Solanaceae and locally infected some species in the families Amaranthaceae, Brassicaceae and Cucurbitaceae. A sensitive and reliable real-time RT-PCR assay was developed using simply prepared crude tissue extract.

引用

页码：353 / 358

页数：6

共 50 条

[1] Molecular and biological properties of tomato necrotic stunt virus and development of a sensitive real-time RT-PCR assay
Rugang Li
Zhangjun Fei
Kai-Shu Ling
Archives of Virology, 2014, 159 : 353 - 358
[2] Development of a real-time RT-PCR assay for detection of tomato mottle mosaic virus
Haghi, Morteza
Ates, Songul Yalcin
Guler, Pakize Gok
Celebi, Oner
Bakirci, Gozde Turkoz
BULGARIAN JOURNAL OF AGRICULTURAL SCIENCE, 2023, 29 (06): : 1154 - 1158
[3] Development of real-time RT-PCR assay for detection of Prunus necrotic ringspot virus in fruit trees
Marbot, S
Salmon, M
Vendrame, M
Huwaert, A
Kummert, J
Dutrecq, O
Lepoivre, P
PLANT DISEASE, 2003, 87 (11) : 1344 - 1348
[4] Development of a real-time RT-PCR SYBR green assay for Tomato ring spot virus in grape
Stewart, Elwin L.
Qu, Xinshun
Overton, Barrie E.
Gildow, Fred E.
Wenner, Nancy G.
Grove, Deborah S.
PLANT DISEASE, 2007, 91 (09) : 1083 - 1088
[5] Development of conventional and Real-time RT-PCR assays for Strawberry necrotic shock virus
Veetil, T. Thekke
Ho, T.
Whitaker, V.
Tzanetakis, I.
PHYTOPATHOLOGY, 2016, 106 (12) : 117 - 117
[6] Development of a graft inoculation method and a real-time RT-PCR assay for monitoring Tomato chlorosis virus infection in tomato
Cevik, Bayram
Klvrak, Hatice
Sahin-Cevik, Mehtap
JOURNAL OF VIROLOGICAL METHODS, 2019, 265 : 1 - 8
[7] Development of a sensitive and quantitative assay for spring viremia of carp virus based on real-time RT-PCR
Yue, Zhiqin
Teng, Yong
Liang, Chengzhu
Xie, Xiayang
Xu, Biao
Zhu, Laihua
Lei, Zhiwen
He, Junqiang
Liu, Zongxiao
Jiang, Yulin
Liu, Hong
Qin, Qiwei
JOURNAL OF VIROLOGICAL METHODS, 2008, 152 (1-2) : 43 - 48
[8] Development of a Real-Time RT-PCR assay for the detection of Cucumber mosaic virus
Jordan, R.
Low, W.
Guaragna, M.
Kamo, K.
PHYTOPATHOLOGY, 2009, 99 (06) : S59 - S59
[9] Development of a TaqMan real-time RT-PCR assay for the detection of rabies virus
Wacharapluesadee, Supaporn
Sutipanya, Jittapawan
Damrongwatanapokin, Sudarat
Phumesin, Patta
Chamnanpood, Pornchai
Leowijuk, Chaweewan
Hemachudha, Thiravat
JOURNAL OF VIROLOGICAL METHODS, 2008, 151 (02) : 317 - 320
[10] Development and evaluation of a real-time RT-PCR assay for Sindbis virus detection
Sane, Jussi
Kurkela, Satu
Levanov, Lev
Nikkari, Simo
Vaheri, Antti
Vapalahti, Olli
JOURNAL OF VIROLOGICAL METHODS, 2012, 179 (01) : 185 - 188

← 1 2 3 4 5 →