Adding new dimensions to laser-scanning fluorescence microscopy

被引：1
|
作者：
De, A. K. [1 ]
Goswami, D. [1 ]
机构：
[1] Indian Inst Technol, Dept Chem, Kanpur 208016, Uttar Pradesh, India
来源：
JOURNAL OF MICROSCOPY | 2009年 / 233卷 / 02期
基金：
英国惠康基金;
关键词：
Confocal microscopy; fluorescence laser scanning microscope; multiphoton microscopy; second-harmonic generation; ultrafast pulsed illumination; 3-dimensional spatial resolution; 2-PHOTON; EXCITATION; PHOTON; CELLS;
D O I：
10.1111/j.1365-2818.2009.03122.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
We describe a novel method of optical imaging by exploiting simple ideas borrowed from pulsed optics. We show that the use of ultrafast pulsed one-photon excitation in laser-scanning fluorescence microscopy dramatically brings together several advantages offered by two widely used present day microscopic techniques, confocal and multi-photon fluorescence microscopy. The method appears as a novel tool in the context of laser-scanning fluorescence microscopy by having a 'built-in' 3D spatial resolution.
引用
收藏
页码：320 / 325
页数：6
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