Quantitation of paralytic shellfish toxins using mouse brain synaptoneurosomes

被引:13
|
作者
David, LS [1 ]
Nicholson, RA [1 ]
机构
[1] Simon Fraser Univ, Dept Biol Sci, Burnaby, BC V5A 1S6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
saxitoxin; mussel extracts; zooplankton extracts; rhodamine 6G fluorescence; membrane potential;
D O I
10.1016/j.chemosphere.2003.10.056
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A membrane potential assay based on synaptoneurosomes prepared from mouse brain was evaluated further for its utility in estimating saxitoxin and related bioactives. Saxitoxin concentrations quantitated in mussel extracts by the synaptoneurosomal technique correlated well with spiked concentrations in these samples (r(2) = 0.995; slope = 1.048). Other experiments found that the synaptoneurosomal assay can detect saxitoxin-like bioactives in zooplankton samples and the concentrations measured were consistent with preliminary estimations of saxitoxin equivalents using the [H-3] saxitoxin receptor binding technique. Veratrine, a mixture of alkaloids that activate sodium channels, had similar potential as a substitute for veratridine in the synaptoneurosomal assay. The results provide additional evidence that the mouse brain synaptoneurosomal membrane potential assay has excellent capability for quantitation of saxitoxin-like activity in shellfish tissues and may also be applied to zooplankton samples. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1315 / 1321
页数:7
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