Detection of Artifical Antigen for Cd(II) by Fluorescence Spectrometry

被引：0
|
作者：
Guo Ming [1 ]
Pang Shuo-Quan [1 ]
Zhang Lu-Ying [1 ]
机构：
[1] Zhejiang Forestry Univ, Dept Chem, Linan 311300, Zhejiang, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2009年 / 37卷 / 07期
关键词：
Cadmium; artificial antigen; fluorescence spectrophotometry; IMMUNOASSAY;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Artificial antigen Cd-IEDTA-BSA (HSA) was successfully synthesized by the bifunctional chelating agent IEDTA (isothiocyanobenzylethyl enediamine tetraacetic acid) coupling with cadmium and protein carrier. The fluroscence characteristics of antigen were determined by fluorescence spectrophotometry and the coupling ratio 11:1 - 16:1 was obtained for the coupling reaction. The folding status of serum albumin during the synthesis process of artificial antigen Cd-IEDTA-BSA (HSA) was analyzed using "fluorescence phase diagram", and the result shows that it was conformed to the "all-or-none" pattern. The polarization fluorescence spectra show that the microenvironments of tryptophan residues in carrier protein have some changes to cause changes in protein conformation.
引用
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页码：1009 / 1013
页数：5
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