Enhanced production of isoflavones by elicitation in hairy root cultures of Soybean

被引：22

作者：

Theboral, Jeevaraj ^{[1
]}

Sivanandhan, Ganeshan ^{[1
]}

Subramanyam, Kondeti ^{[1
]}

Arun, Muthukrishnan ^{[2
]}

Selvaraj, Natesan ^{[3
]}

Manickavasagam, Markandan ^{[1
]}

Ganapathi, Andy ^{[1
]}

机构：

[1] Bharathidasan Univ, Sch Biotechnol, Dept Biotechnol & Genet Engn, Tiruchirappalli 620024, Tamil Nadu, India

[2] SRM Univ, Dept Genet Engn, Sch Bioengn, Kattankulathur 603203, Tamil Nadu, India

[3] Periyar EVR Coll, Dept Bot, Plant Tissue Culture Lab, Tiruchirappalli 620023, Tamil Nadu, India

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2014年 / 117卷 / 03期

关键词：

Elicitors; Hairy root culture; Isoflavones; Sonication; Soybean; Vacuum infiltration; GROWTH; GENES;

D O I：

10.1007/s11240-014-0450-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A combined treatment of sonication (2 min) and vacuum infiltration (2 min) stimulated isoflavones production of 75.26 mg g(-1) DW which was 15.11-fold higher than control hairy root line at optimal harvest time of 40 days. Addition of MeJ at 100 mu Mconcentration with 72 h exposure time on 30 day-old hairy root culture further enhanced total isoflavones production of 53.16 mg g(-1) DW (10.67-fold) and SA at 200 mu M concentration with 96 h exposure period enhanced the production of isoflavones (28.79 mg g(-1) DW; 5.78-fold). MeJ-treated hairy roots reduced biomass accumulation whereas sonication, vacuum infiltration and SA did not exhibit a negative effect on biomass growth.

引用

页码：477 / 481

页数：5

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