Hepatic carnitine palmitoyltransferase activity in cattle

被引：4

作者：

Mizutani, H ^{[1
]}

Sako, T ^{[1
]}

Takemura, N ^{[1
]}

Koyama, H ^{[1
]}

Yamaguchi, M ^{[1
]}

Motoyoshi, S ^{[1
]}

机构：

[1] OHIO STATE UNIV,DEPT VET BIOSCI,MUSCLE BIOL LAB,COLUMBUS,OH 43210

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1997年 / 59卷 / 11期

关键词：

carnitine palmitoyltransferase; cattle; fluorometric assay;

D O I：

10.1292/jvms.59.1067

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A fluorometric assay for the determination of hepatic carnitine palmitoyltransferase (CPT) activity was slightly modified for use with cattle samples. With this assay, the K-m value was 0.56 +/- 0.10 mM with respect to L-carnitine (mean +/- SD, n=4) and was 9.6 +/- 2.2 mu M (n=3) with respect to palmitoyl-CoA. The average hepatic CPT activity was 33.6 +/- 2.0 mu mol CoASH/min/g protein in 38 healthy cattle and was similar in both sexes and among breeds. Hepatic CPT activity showed no correlation with serum phospholipid, free fatty acid, triglyceride or total cholesterol concentrations.

引用

页码：1067 / 1069

页数：3

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