A gold nanoparticle-based four-color proximity immunoassay for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification

被引：19

作者：

Xu, Jiayao ^{[1
]}

Shi, Ming ^{[2
]}

Chen, Wenting ^{[1
]}

Huang, Yong ^{[1
]}

Fang, Lina ^{[1
]}

Yao, Lifang ^{[1
]}

Zhao, Shulin ^{[1
]}

Chen, Zhen-Feng ^{[1
]}

Liang, Hong ^{[1
]}

机构：

[1] Guangxi Normal Univ, Coll Chem & Pharm, State Key Lab Chem & Mol Engn Med Resources, Guilin 541004, Guangxi, Peoples R China

[2] Guilin Normal Coll, Dept Chem & Pharm, Guilin, Guangxi, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 22期

关键词：

LIVING CELLS; HOMOGENEOUS IMMUNOASSAY; CANCER; ASSAY; FLUORESCENCE; BINDING; LIGATION; PROBES; NANOCLUSTERS; APTASENSOR;

D O I：

10.1039/c7cc09404c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel gold nanoparticle-based four-color fluorescence proximity immunoassay is developed for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification. This assay could detect proteins with detection limits down to pg mL(-1) and a 5-log dynamic range. Furthermore, it is suitable for rapid, direct quantification of multiple proteins in biological samples.

引用

页码：2719 / 2722

页数：4

共 14 条

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