Synthesis and characterization of multifunctional organic-inorganic composite hydrogel formed with tissue-adhesive property and inhibiting infection

Organic-inorganic composite hydrogel materials have been widely studied. In order to expand the application of organic-inorganic composite hydrogel materials, in this work, we prepared a viscous hydrogel with antibacterial properties (OSA-GelDA@ACP/DA/Ag). First, we used polydopamine coating to deposit elemental silver on the surface of amorphous calcium phosphate (ACP) particles to prepare ACP/DA/Ag particles. Next, dopamine was grafted on the gelatin molecular chain by EDC/NHS to activate the carboxyl group to obtain dopamine-modified gelatin (GelDA). The content of DA in GelDA is about 14.09% by standard curve method. Then, the Schiff base reaction took place between the amino group on the GelDA molecular chain and the aldehyde group on the OSA molecular chain, and an OSA-GelDA viscous hydrogel was prepared. Finally, by changing the content of ACP/DA/Ag3 particles added to the OSA-GelDA gel, the corresponding performance of material was investigated. The results show that the introduction of dopamine provides wet viscosity for the hydrogel, and the ACP/DA/Ag3 particles introduced in the viscous hydrogel provide antibacterial properties. This hydrogel with antibacterial and wet viscosity is expected to become an injectable bone repair material for clinical use.