Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage

被引:19
|
作者
Hamidian, Mohammad [1 ]
Blasco, Lucia [2 ]
Tillman, Lauren N. [1 ]
To, Joyce [1 ]
Tomas, Maria [2 ]
Myers, Garry S. A. [1 ]
机构
[1] Univ Technol Sydney, iThree Inst, Ultimo, NSW 2007, Australia
[2] Univ A Coruna UDC, Hosp A Coruna CHUAC, Microbiol Dept, Res Inst Biomed Coruna INIBIC, La Coruna 15495, Spain
基金
澳大利亚研究理事会;
关键词
Acinetobacter baumannii ATCC 19606; complete genome; plasmid; antibiotic resistance; ST52 and Multi-Locus Sequence Typing (MLST); CONJUGATIVE PLASMID; RESISTANCE; COLISTIN; AGAR;
D O I
10.3390/microorganisms8121851
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Acinetobacter baumannii isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of A. baumannii, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn6551. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type.
引用
收藏
页码:1 / 22
页数:22
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