Transient expressions of synthetic biology in plants

被引:86
|
作者
Sainsbury, Frank [1 ]
Lomonossoff, George P. [2 ]
机构
[1] Univ Queensland, Australian Inst Bioengn & Nanotechnol, Ctr Biomol Engn, St Lucia, Qld 4072, Australia
[2] John Innes Inst, Dept Biol Chem, Norwich NR4 7UH, Norfolk, England
基金
英国生物技术与生命科学研究理事会;
关键词
COWPEA MOSAIC-VIRUS; HETEROLOGOUS PROTEINS; HIGH-LEVEL; SYSTEM; IDENTIFICATION; TRANSLATION; PARTICLES; VECTORS; QUICK;
D O I
10.1016/j.pbi.2014.02.003
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Recent developments in transient expression methods have enabled the efficient delivery and expression of multiple genes within the same plant cell over a timescale of days. In some cases, the vectors deployed can be fine-tuned to allow differential expression of the various genes. This has opened the way to the deployment of transient expression for such applications as the production of macromolecular complexes and the analysis and manipulation of metabolic pathways. The ability to observe the effect of gene expression in a matter of days means that transient expression is becoming the method of choice for many plant-based synthetic biology applications.
引用
收藏
页码:1 / 7
页数:7
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