Recent developments in transient expression methods have enabled the efficient delivery and expression of multiple genes within the same plant cell over a timescale of days. In some cases, the vectors deployed can be fine-tuned to allow differential expression of the various genes. This has opened the way to the deployment of transient expression for such applications as the production of macromolecular complexes and the analysis and manipulation of metabolic pathways. The ability to observe the effect of gene expression in a matter of days means that transient expression is becoming the method of choice for many plant-based synthetic biology applications.
机构:
Mitsubishi Chem Res Corp, 16-1 Samon Cho,Sinjuku Ku, Tokyo 1060017, JapanMitsubishi Chem Res Corp, 16-1 Samon Cho,Sinjuku Ku, Tokyo 1060017, Japan
Hayakawa, Takahiko
Suzuki, Hayato
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Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukisamu Higashi 2-17-2-1, Sapporo, Hokkaido 0628517, JapanMitsubishi Chem Res Corp, 16-1 Samon Cho,Sinjuku Ku, Tokyo 1060017, Japan
Suzuki, Hayato
Yamamoto, Hiroshi
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Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Cent 6,Higashi 1-1-1, Tsukuba, Ibaraki 3058566, JapanMitsubishi Chem Res Corp, 16-1 Samon Cho,Sinjuku Ku, Tokyo 1060017, Japan
Yamamoto, Hiroshi
Mitsuda, Nobutaka
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Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukisamu Higashi 2-17-2-1, Sapporo, Hokkaido 0628517, Japan
Natl Inst Adv Ind Sci & Technol, Global Zero Emiss Res Ctr, Cent 6,Higashi 1-1-1, Tsukuba, Ibaraki 3058566, JapanMitsubishi Chem Res Corp, 16-1 Samon Cho,Sinjuku Ku, Tokyo 1060017, Japan
机构:
Univ Western Australia, Australian Res Council Ctr Excellence Plant Energ, Crawley, WA 6009, AustraliaUniv Western Australia, Australian Res Council Ctr Excellence Plant Energ, Crawley, WA 6009, Australia
Small, Ian
Puchta, Holger
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Karlsruhe Inst Technol, Bot Inst 2, D-76049 Karlsruhe, GermanyUniv Western Australia, Australian Res Council Ctr Excellence Plant Energ, Crawley, WA 6009, Australia
机构:
Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Sapporo, Hokkaido 0628517, Japan
Natl Inst Adv Ind Sci & Technol, Global Zero Emiss Res Ctr GZR, Tsukuba 3058569, JapanNatl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Sapporo, Hokkaido 0628517, Japan
Mitsuda, Nobutaka
Seki, Hikaru
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Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka, Japan
Osaka Univ, Inst Open & Transdisciplinary Res Initiat, Ind Biotechnol Initiat Div, Suita, Osaka 5650871, JapanNatl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Sapporo, Hokkaido 0628517, Japan
Seki, Hikaru
Shoji, Tsubasa
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Univ Toyama, Inst Nat Med, Toyama 9300194, Japan
RIKEN Ctr Sustainable Resource Sci, Yokohama, Kanagawa 2300045, JapanNatl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Sapporo, Hokkaido 0628517, Japan
Shoji, Tsubasa
Hirai, Masami Yokota
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RIKEN Ctr Sustainable Resource Sci, Yokohama, Kanagawa 2300045, Japan
Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Biosci, Nagoya, Aichi 4648601, JapanNatl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Sapporo, Hokkaido 0628517, Japan