A Temperature Control System for Polymerase Chain Reaction Machine for Cell-Systematic Evolution of Ligands by Exponential Enrichment-Based Automated Screening of Nucleic Acid Aptamers

被引：11
|
作者：
Zhou, Xin [1 ]
Zhang, Chuanxiang [3 ]
Jiang, Hui [1 ]
Wang, Chao [1 ]
Wang, Zunliang [1 ]
Lu, Chuan [1 ]
He, Nongyue [1 ,2 ]
机构：
[1] Southeast Univ, Natl Demonstrat Ctr Expt Biomed Engn Educ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
[2] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Econ Forest Cultivat & Utilizat Collaborat Innova, Zhuzhou 412007, Peoples R China
[3] Hunan Univ Technol, Sch Packaging & Mat Engn, Zhuzhou 412007, Hunan, Peoples R China
来源：
NANOSCIENCE AND NANOTECHNOLOGY LETTERS | 2018年 / 10卷 / 04期
基金：
国家重点研发计划;
关键词：
Fuzzy Control; PID Control; Cell-SELEX; PCR Thermal Cycling; Aptamers Screening; Molecular Diagnostics; REAL-TIME PCR; BOVINE SERUM-ALBUMIN; MEDIATED ISOTHERMAL AMPLIFICATION; TETRAHEDRAL DNA NANOSTRUCTURES; GOLD MAGNETIC NANOPARTICLES; NUMBER VARIATION ANALYSIS; DUAL-COLOR HYBRIDIZATION; LIQUID HANDLING-SYSTEM; CARBON-PASTE ELECTRODE; TARGETED DRUG-DELIVERY;
D O I：
10.1166/nnl.2018.2695
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
In this work, we designed and developed a temperature control system for the polymerase chain reaction (PCR) machine for cell-SELEX-based automated nucleic acid aptamers screening. A 500-mu L PCR reaction chamber was designed for the temperature control system according to the cell-SELEX screening protocol. For the large-volume PCR reaction, a cascade control scheme was adopted for fast and accurate temperature control by combining a fuzzy PID controller with a PI controller. The experimental results showed that the system presented in this paper can implement effective PCR temperature control to meet the design requirements for automation of aptamer screening.
引用
收藏
页码：596 / 602
页数：7
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