An efficient protocol for callus induction in Poeciloneuron indicum Bedd.: An endemic medicinal plant of Western Ghats

Poeciloneuron indicum Bedd. is an endemic plant of Clusiaceae, useful as both in medicine as well as in timber. Among the tested culture medias, MS and WPM are proved to be potential media to induce callus 95% of callusing was observed in MS medium supplemented with 1.5 ppm of TDZ alone, followed by 94% of callusing with 1.5 ppm of TDZ and 0.5 ppm of 2, 4-D in combination. WPM was less potent compared to MS media and resulted with 65% of callusing which was supplemented with 1.5 ppm of 2,4-D and 0.5 ppm of TDZ, followed by 59% callusing when supplemented with TDZ alone. Leaf explants were categorized in to L1 (brown young leaves), L2 (pale green leaves) and L3 (green) among which, L2 has shown better callusing, followed by L1, however no response was observed with L3. Compared to leaf and young stem, leaf explants has shown better response with greater amount of callusing in the above-mentioned growth hormone. Callus observed was compact and hard, faded white to pale brownish in colour. This is the first report for callus induction on the selected plant species.