Highly sensitive real-time detection of DNA hybridization by using nanoporous waveguide fluorescence spectroscopy

被引：13

作者：

Fan, Yong ^{[1
,2
]}

Hotta, Kazuhiro ^{[3
]}

Yamaguchi, Akira ^{[4
,5
]}

Ding, Yu ^{[1
,2
]}

He, Yonghong ^{[1
]}

Teramae, Norio ^{[3
]}

Sun, Shuqing ^{[1
]}

Ma, Hui ^{[1
,2
]}

机构：

[1] Tsinghua Univ, Grad Sch Shenzhen, Shenzhen Key Lab Minimal Invas Med Technol, Shenzhen 518055, Peoples R China

[2] Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China

[3] Tohoku Univ, Grad Sch Sci, Dept Chem, Aoba Ku, Sendai, Miyagi 9808578, Japan

[4] Ibaraki Univ, Coll Sci, Bunkyo Ku, Mito, Ibaraki 3108512, Japan

[5] Ibaraki Univ, Frontier Res Ctr Appl Atom Sci, Bunkyo Ku, Mito, Ibaraki 3108512, Japan

来源：

APPLIED PHYSICS LETTERS | 2014年 / 105卷 / 03期

关键词：

ENHANCED FLUORESCENCE; OPTIMIZATION; TEMPLATES; MEMBRANES;

D O I：

10.1063/1.4890984

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

In this Letter, we report highly sensitive fluorescence spectroscopy using a nanoporous waveguide (NPWG) comprising a porous anodic alumina (PAA) layer and an Al layer. Simulations show that the TE0 waveguide mode excited in the PAA layer produces an electromagnetic field whose intensity is 40-fold higher than that of the incident light, and which yields enhanced intensity when used to excite fluorophores. We demonstrate the sensing ability of the NPWG by incorporating it into a fluorescent sensor to monitor duplex DNA formation in real-time, with a detection limit as low as 20 pM. (C) 2014 AIP Publishing LLC.

引用

页数：4

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