Alginate molecular mass produced by Azotobacter vinelandii in response to changes of the O2 transfer rate in chemostat cultures

被引：12

作者：

Diaz-Barrera, A. ^{[1
]}

Silva, P. ^{[1
]}

Avalos, R. ^{[1
]}

Acevedo, F. ^{[1
]}

机构：

[1] Pontificia Univ Catolica Valparaiso, Escuela Ingn Bioquim, Valparaiso 4059, Chile

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 06期

关键词：

Alginate; Chemostat; Molecular mass; Oxygen transfer rate; OXYGEN-TRANSFER RATE; PSEUDOMONAS-AERUGINOSA; WEIGHT; BIOSYNTHESIS;

D O I：

10.1007/s10529-009-9949-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O-2 transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g l(-1)) was observed. At a constant growth rate (0.1 h(-1)), the mean molecular mass of the alginate was strongly influenced by changes in the OTR, varying from 860 to 1,690 kDa. These results support a possible relationship between alginate polymerization-depolymerization process and the O-2 uptake rate.

引用

页码：825 / 829

页数：5

共 16 条

[1] Alginate molecular mass produced by Azotobacter vinelandii in response to changes of the O2 transfer rate in chemostat cultures
A. Díaz-Barrera
P. Silva
R. Ávalos
F. Acevedo
Biotechnology Letters, 2009, 31 : 825 - 829
[2] The oxygen transfer rate influences the molecular mass of the alginate produced by Azotobacter vinelandii
A. Díaz-Barrera
C. Peña
E. Galindo
Applied Microbiology and Biotechnology, 2007, 76 : 903 - 910
[3] The oxygen transfer rate influences the molecular mass of the alginate produced by Azotobacter vinelandii
Diaz-Barrera, A.
Pena, C.
Galindo, E.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 76 (04) : 903 - 910
[4] Specific growth rate determines the molecular mass of the alginate produced by Azotobacter vinelandii
Priego-Jimenéz, R
Peña, C
Ramírez, OT
Galindo, E
BIOCHEMICAL ENGINEERING JOURNAL, 2005, 25 (03) : 187 - 193
[5] Manipulating the molecular weight of alginate produced by Azotobacter vinelandii in continuous cultures
Diaz-Barrera, Alvaro
Silva, Paulina
Berrios, Julio
Acevedo, Fernando
BIORESOURCE TECHNOLOGY, 2010, 101 (23) : 9405 - 9408
[6] The viscosifying power, degree of acetylation and molecular mass of the alginate produced by Azotobacter vinelandii in shake flasks are determined by the oxygen transfer rate
Pena, C.
Galindo, E.
Buechs, J.
PROCESS BIOCHEMISTRY, 2011, 46 (01) : 290 - 297
[7] Changes in alginate molecular mass distributions, broth viscosity and morphology of Azotobacter vinelandii cultured in shake flasks
Pena, C
Campos, N
Galindo, E
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1997, 48 (04) : 510 - 515
[8] Changes in alginate molecular mass distributions, broth viscosity and morphology of Azotobacter vinelandii cultured in shake flasks
C. Peña
N. Campos
E. Galindo
Applied Microbiology and Biotechnology, 1997, 48 : 510 - 515
[9] Influence of the oxygen transfer rate (OTR) on the alginate production and molecular mass of the polymer in cultures of Azotobacter vinelandii under oxygen-limited and non-oxygen-limited conditions
Pena-Malacara, C.
Lozano, E.
Galindo, E.
NEW BIOTECHNOLOGY, 2009, 25 : S227 - S227
[10] Evolution of the specific power consumption and oxygen transfer rate in alginate-producing cultures of Azotobacter vinelandii conducted in shake flasks
Pena, Carlos
Peter, Cyril P.
Buechs, Jochen
Galindo, Enrique
BIOCHEMICAL ENGINEERING JOURNAL, 2007, 36 (02) : 73 - 80

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