Biochemical profiles following flunixin meglumine administration in combination with hypertonic saline during endotoxic shock in buffalo calves

Endotoxic shock was produced in 5 apparently healthy male buffalo calves aged between 4 months and I year by U v infusion of Escherichia coli endotoxin @ 5 mu g/kg BW/h for 3 h. The animals were further observed for 7 days. A general hypoproteinemia was observed during endotoxin infusion along with significant hypoalbuminemia, hypoglycemia, hypofibrinogenemia, decrease in plasma globulin,creatinine while plasma blood urea nitogen, sodium, potassium, and chloride showed nonsignificant alterations during endotoxin infusion for 3 h. All the endotoxemic buffalo calves were infused i/v hypertonic saline solution (7.2% NaCl acq.) @ 4 ml/kg BW in 6.5 min followed by flunixin meglumine @ 1.1 mg/kg BW as one time infusion and plasma proteins showed a significant decrease at 4 h but increased significantly on day 2 while albumin showed a declining trend throughout the observation period with a significant fall at 4 h of start of endotoxin infusion. While plasma globulin decreased, plasma fibrin increased significantly on second and third day. Hypoglycemia continued from day 1-6 accompanied by a nonsignificant fall in BUN within normal range throughout the 7 day observation period. The plasma creatinine increased significantly at 5 and 7 h of day I and on day 2 of observation. Plasma sodium potassium and chloride did not alter significantly indicating that this treatment combination does not greatly affect the biochemical profiles during bovine endotoxemia.