Expression of mouse RNase MRP RNA in human embryonic kidney 293 cells

被引：9

作者：

Rossmanith, W ^{[1
]}

Bettinger, E ^{[1
]}

Cerni, C ^{[1
]}

Karwan, RM ^{[1
]}

机构：

[1] UNIV VIENNA, INST TUMORBIOL KREBSFORSCH, ARBEITSGRP TUMORVIROL, A-1090 VIENNA, AUSTRIA

来源：

MOLECULAR BIOLOGY REPORTS | 1997年 / 24卷 / 04期

基金：

奥地利科学基金会;

关键词：

gene expression; ribonucleoprotein; RNase MRP; RNase P; transcription;

D O I：

10.1023/A:1006882704481

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the expression of mouse RNase MRP RNA in human embryonic kidney 293 cells upon DNA transfection. Stable cell lines were selected by cotransfection with a neo(r) gene. Transcription of wild-type and deletion mutants of MRP RNA and ribonucleoprotein formation were assessed by RNase protection and immunoprecipitation experiments. Mouse MRP RNA as expressed in 293 cells readily associates with human proteins to form a chimeric Th ribonucleoprotein. 5' truncated MRP RNAs, however, failed to associate with Th antigen(s) and deletion of the 3' sequences of MRP RNA greatly reduced the expression in stable as well as in transient transfectants.

引用

页码：221 / 230

页数：10

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