Mapping novel traits by array-assisted bulk segregant analysis in Saccharomyces cerevisiae

被引：72

作者：

Brauer, Matthew J.

Christianson, Cheryl M.

Pai, Dave A.

Dunham, Maitreya J.

机构：

[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

[2] Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08544 USA

来源：

GENETICS | 2006年 / 173卷 / 03期

关键词：

D O I：

10.1534/genetics.106.057927

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We demonstrate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genotyping pooled segregants. We apply a probabilistic model to the progeny of a single cross and as little as two microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effect quantitative trait locus.

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收藏

页码：1813 / 1816

页数：4

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