Fluconazole and Voriconazole Resistance in Candida parapsilosis Is Conferred by Gain-of-Function Mutations in MRR1 Transcription Factor Gene

被引：39
|
作者：
Branco, Joana [1 ]
Silva, Ana P. [1 ,2 ]
Silva, Raquel M. [3 ,4 ,5 ]
Silva-Dias, Ana [1 ,2 ]
Pina-Vaz, Cidalia [1 ,2 ]
Butler, Geraldine [6 ]
Rodrigues, Acacio G. [1 ,2 ]
Miranda, Isabel M. [1 ,2 ]
机构：
[1] Univ Porto, Fac Med, Dept Microbiol, P-4100 Porto, Portugal
[2] Univ Porto, Fac Med, Ctr Hlth Technol & Serv Res CINTESIS, P-4100 Porto, Portugal
[3] Univ Aveiro, Inst Biomed iBiMED, P-3800 Aveiro, Portugal
[4] Univ Aveiro, IEETA, P-3800 Aveiro, Portugal
[5] Univ Porto, IPATIMUP, Inst Mol Pathol & Immunol, P-4100 Porto, Portugal
[6] Univ Coll Dublin, Conway Inst, UCD Sch Biomol & Biomed Sci, Dublin 2, Ireland
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2015年 / 59卷 / 10期
关键词：
ANTIFUNGAL DRUG-RESISTANCE; UP-REGULATION; MECHANISMS; OVEREXPRESSION; HETEROZYGOSITY; ORTHOPSILOSIS; METAPSILOSIS; REGULATOR;
D O I：
10.1128/AAC.00842-15
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Candida parapsilosis is the second most prevalent fungal agent causing bloodstream infections. Nevertheless, there is little information about the molecular mechanisms underlying azole resistance in this species. Mutations (G1747A, A2619C, and A3191C) in the MRR1 transcription factor gene were identified in fluconazole- and voriconazole-resistant strains. Independent expression of MRR1 genes harboring these mutations showed that G1747A (G583R) and A2619C (K873N) are gain-of-function mutations responsible for azole resistance, the first described in C. parapsilosis.
引用
收藏
页码：6629 / 6633
页数：5
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