Construction and performance of two-dimensional separation platform of coupling capillary isoelectric focusing-zone electrophoresis for proteins

被引：0

作者：

Liu, HC ^{[1
]}

Yang, C ^{[1
]}

Zhang, WB ^{[1
]}

Zhang, YK ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2004年 / 25卷 / 08期

关键词：

microialysis hollow fiber membrane interface; two-dimensional separation platform; capillary isoelectric focusing; capillary zone electrophoresis;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A two-dimensional separation platform of capillary isoelectric focusing (CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of fabrication, speed of mass delivering and convenience of column switching. Chrome c was selected to demonstrate the 2D separation system's feasibility and resolving performance. The 2D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.

引用

页码：1451 / 1453

页数：3

共 7 条

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[6] On-line hyphenation of capillary isoelectric focusing and capillary gel electrophoresis by a dialysis interface
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Liu, HC
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[J]. ANALYTICAL CHEMISTRY, 2003, 75 (02) : 215 - 218
[7] Zhang SY, 2002, CHEM J CHINESE U, V23, P1246

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