Flectobacillus fontis sp nov., isolated from a freshwater spring

A bacterial strain, designated MIB-4(T), was isolated from a freshwater spring in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain MIB-4(T) were Gram-staining-negative, aerobic, non-motile rods that formed pale pink colonies. Growth occurred at 15-30 C-circle (optimum, 25 C-circle), at pH 7-8 (optimum, pH 7) and with 0-0.5% NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MIB-4(T) belonged to the genus Flectobacillus and was most closely related to Flectobacillus lacus CL-GP79(T) with sequence similarity of 98.7 %. Strain MIB-4(T) contained C-16 : 1 omega 5c, iso-C-15 : 0 and summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, six unidentified aminophospholipids, one unidentified glycolipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content of the genomic DNA was 37.6 mol%. The DNA-DNA hybridization value for strain MIB-4(T) with F. lacus CL-GP79(T) was less than 32 %. On the basis of the phylogenetic inference and phenotypic data, strain MIB-4(T) should be classified as a representative of a novel species, for which the name Flectobacillus fontis sp. nov. is proposed. The type strain is MIB-4(T) (= BCRC 80926(T) = LMG 29292(T) = KCTC 33763(T)).