DEVELOPMENT OF AN INFANT FORMULA HIGH IN ALPHA-LACTALBUMIN WITH ONLY A2 BETA-CASEIN BY SPRAY DRYING, DESIGNED TO RESEMBLE THE PROTEIN COMPOSITION OF HUMAN MILK

In this work, an infant formula enriched in alpha-lactalbumin and whose beta-casein fraction contains only A(2) beta-casein was development by spray drying. Experimental infant formula (EF) was designed to closely resemble the protein composition of human milk. Response surface method (RSM) with two factors: total solids and inlet air temperature was used for the optimization of the spray drying process. Response variables were solubility, moisture content, luminance and peroxide value. To define optimal drying conditions, a stability test was performed for two treatments (120 degrees C, 40 wt. % and 170 degrees C, 30 wt. %), which were obtained from the RSM. The treatment with 170 degrees C and 30 wt. % concentration of total solids was selected as the best alternative for the spray drying process. The protein composition in the EF was 37.1% casein, 23.1% alpha-lactalbumin and 21.3% beta-lactoglobulin. A decrease of 6% in /3-lactoglobulin increased the relative proportion of a-lactalbumin, which favoured the similarity of the EF to breast milk. The homology of the beta-caseins with the A(2) beta-casein isoform was 99.82%.