Lysinibacillus composti sp nov., isolated from compost

A Gram-negative, motile, rod-shaped, endospore-forming bacterial strain, designated as NCCP-36(T), was isolated from the compost of fruit and vegetable wastes. The strain NCCP-36(T) grew within a temperature range of 10-45 C-au < (optimum 28 C-au <) and a pH range of 6.5-8.5 (optimum 7.0), and its cells tolerated < 50 mM boron (optimum growth without boron) and 0-5 % NaCl (w/v) in tryptic soya broth medium. Based on comparative analysis of 16S rRNA gene sequence, strain NCCP-36(T) showed the highest similarity to Lysinibacillus sinduriensis BLB-1(T) (97.52 %) and L. xylanilyticus XDB9(T) (96.96 %), and < 97 % similarity with other closely related taxa. However, DNA-DNA relatedness between strain NCCP-36(T) and the closely related type strains of genus Lysinibacillus was a parts per thousand currency sign37 %. Phylogenetic and chemotaxonomic analyses [major polar lipids: diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phospholipids; predominant menaquinone: MK-7; major cellular fatty acids: iso-C-15:0, antieso-C-15:0, and iso-C-16:0; DNA G+C contents: 37 mol %; Lys-Asp (type A4 alpha) in cell-wall peptidoglycans as diagnostic amino acids] also support the affiliation of strain NCCP-36(T) to genus Lysinibacillus. Based upon DNA-DNA relatedness as well as distinctive chemotaxonomic, phylogenetic, and genotypic data, we conclude that strain NCCP-36(T) belongs to a novel species of genus Lysinibacillus, for which the name Lysinibacillus composti sp. nov. is proposed. The type strain is NCCP-36(T) (JCM 18777(T) = KCTC 13796(T) = DSMZ 24785(T)).