Isolation and characterization of bacteriophage SPI1, which infects the activated-sludge-foaming bacterium Skermania piniformis

被引：13

作者：

Dyson, Z. A. ^{[1
]}

Tucci, J. ^{[1
]}

Seviour, R. J. ^{[2
]}

Petrovski, S. ^{[2
]}

机构：

[1] La Trobe Inst Mol Sci, Bendigo, Vic 3550, Australia

[2] La Trobe Univ, Dept Physiol Anat & Microbiol, Bundoora, Vic 3086, Australia

来源：

ARCHIVES OF VIROLOGY | 2016年 / 161卷 / 01期

关键词：

Phage; Bacteriophage; Skermania piniformis; PTLO; Activated sludge foaming; Bio-control; Wgs; Phage therapy; GENOME SEQUENCE; MYCOBACTERIOPHAGE GENOMES; ESCHERICHIA-COLI; RHODOCOCCUS-EQUI; DNA; PLANTS; GORDONIA; GENES; ENDONUCLEASE; BIOCONTROL;

D O I：

10.1007/s00705-015-2631-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Foaming in activated sludge plants is a worldwide problem commonly caused by proliferation of bacteria of the order Corynebacteriales. These include Skermania piniformis, a filamentous bacterium that has been documented to be a major cause of foaming globally, and particularly in Australian treatment plants. Phage SPI1 is the first phage that was isolated and shown to infect this organism. It targets seven of the nine strains of S. piniformis held in our culture collection, but none of the other 73 mycolata strains of different genera, mostly isolated from wastewater, against which it was tested. Phage SPI1 is a member of the family Siphoviridae and has a circularly permuted dsDNA genome of 55,748 bp with a G+C content of 67.8 mol %. It appears to be obligatorily lytic, with no evidence of genes related to a lysogenic mode of existence.

引用

页码：149 / 158

页数：10