A flow-through enzymatic microreactor immobilizing lipase based on layer-by-layer method for biosynthetic process: Catalyzing the transesterification of soybean oil for fatty acid methyl ester production

被引：29

作者：

Bi, Yicheng ^{[1
,2
]}

Zhou, Hua ^{[1
]}

Jia, Honghua ^{[2
]}

Wei, Ping ^{[2
]}

机构：

[1] Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing 211816, Jiangsu, Peoples R China

[2] Nanjing Tech Univ, Biotechnol & Pharmarceut Engn, Nanjing, Jiangsu, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2017年 / 54卷

基金：

中国国家自然科学基金;

关键词：

Immobilized enzyme microreactor; Layer-by-layer assembly; Biodiesel; Polydopamine; BIODIESEL; CAPILLARY; REACTOR; POLYDOPAMINE; FABRICATION; SEPARATION; MICROCHIP; SUPPORT; ENZYMES;

D O I：

10.1016/j.procbio.2016.12.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and convenient method was proposed in this paper to develop a flow-through enzymatic micro reactor made from polytetrafluoroethylene (PTFE). It consisted of the polydopamine layer (functioned as a primer) and layer by layer (LBL) coatings composed of polyethylenimine (PEI) and lipase. The multiple deposition of PEI and lipase was the key factor of increasing the enzyme loading on microreactor. After 8 PEI/lipase layers, enzyme loading on the inner surface of 5-m microchannel reached a maximum (350 mu g to 400 mu g), compared With approximately 20 mu g in single layer. Microreactor with higher enzyme loading was successfully applied on transesterification of soybean oil for effective fatty acid methyl ester (FAME, biodiesel) production. A 95.2% conversion rate of biodiesel can be achieved in 53 min under optimized conditions, instead of a couple of hours in the traditional batch reaction. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：73 / 80

页数：8