Enhanced cell transplantation: preventing apoptosis increases cell survival and ventricular function

被引:40
|
作者
Nakamura, Yoshinobu [1 ]
Yasuda, Tamotsu [1 ]
Weisel, Richard D. [1 ]
Li, Ren-Ke [1 ]
机构
[1] Univ Toronto, Toronto Gen Hosp, Toronto Gen Res Inst, Div Cardiac Surg,Dept Surg, Toronto, ON M5G 1L7, Canada
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2006年 / 291卷 / 02期
关键词
cell therapy; Bcl-2; heat shock; ventricular modulation; angiogenesis;
D O I
10.1152/ajpheart.00155.2006
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Cell transplantation prevents cardiac dysfunction after myocardial infarction. However, because most implanted cells are lost to ischemia and apoptosis, the benefits of cell transplantation on heart function could be improved by increasing cell survival. To examine this possibility, male Lewis rat aortic smooth muscle cells (SMCs; 4 x 10(6)) were pretreated with antiapoptotic Bcl-2 gene transfection or heat shock and then implanted into the infarcted myocardium of anesthetized, syngenic female rats (n = 23 per group). On the first day after transplantation, apoptotic SMCs were quantified by using transferase-mediated dUTP nick-end labeling staining. On days 7 and 28, grafted cell survival was quantified by using real-time PCR, and heart function was assessed with the use of echocardiography and the Langendorff apparatus. SMCs given antiapoptotic pretreatments exhibited improvements in each measure relative to controls. Apoptosis was reduced in Bcl-2-treated cells relative to all other groups (P < 0.05), whereas survival (P < 0.01) was increased. Heat shock also significantly decreased apoptosis and increased survival relative to control groups (P < 0.05 for group effect), although these effects were less pronounced than in the Bcl-2-treated group. Further, scar areas were reduced in both Bcl-2- and heat shock-treated groups relative to controls (P < 0.05), and fractional area change and cardiac function were greater (P < 0.05 for both measures). These results indicate that antiapoptosis pretreatments reduced grafted SMC loss after transplantation and enhanced grafted cell survival and ventricular function, which was directly related (r = 0.72; P = 0.002) to the number of surviving engrafted cells.
引用
收藏
页码:H939 / H947
页数:9
相关论文
共 50 条
  • [1] Inhibition of "tissue" transglutaminase increases cell survival by preventing apoptosis
    Oliverio, S
    Amendola, A
    Rodolfo, C
    Spinedi, A
    Piacentini, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (48) : 34123 - 34128
  • [2] Mast Cell Stabilization Improves Survival by Preventing Apoptosis in Sepsis
    Ramos, Laura
    Pena, Geber
    Cai, Bolin
    Deitch, E. A.
    Ulloa, Luis
    JOURNAL OF IMMUNOLOGY, 2010, 185 (01): : 709 - 716
  • [3] Cell transplantation to improve ventricular function in the failing heart
    Tang, GHL
    Fedak, PWM
    Yau, TM
    Weisel, RD
    Kulik, A
    Mickle, DAG
    Li, RK
    EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY, 2003, 23 (06) : 907 - 916
  • [4] Cell transplantation in dilated cardiomyopathy preserved ventricular function
    Ohno, N
    Li, RK
    Weisel, RD
    Mickle, DAG
    Fedak, PWM
    JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2001, 33 (06) : A86 - A86
  • [5] Multipotent adult progenitor cell transplantation increases vascularity and improves left ventricular function after myocardial infarction
    Pelacho, Beatriz
    Nakamura, Yasuhiro
    Zhang, Jianyi
    Ross, Jeff
    Heremans, Yves
    Nelson-Holte, Molly
    Lemke, Brad
    Hagenbrock, Julianna
    Jiang, Yuehua
    Prosper, Felipe
    Luttun, Aernout
    Verfaillie, Catherine M.
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2007, 1 (01) : 51 - 59
  • [6] Not chronic exercise but acute exercise is related with increased cell survival, enhanced cell proliferation and decreased cell apoptosis
    Postula, M.
    Wicik, Z.
    Eyileten, C.
    Soplinska, A.
    Czajka, P.
    Nowak, A.
    Jarosz-Popek, J.
    Malek, L.
    EUROPEAN HEART JOURNAL, 2020, 41 : 2880 - 2880
  • [7] MAST CELL APOPTOSIS AND SURVIVAL
    Ekoff, Maria
    Nilsson, Gunnar
    MAST CELL BIOLOGY: CONTEMPORARY AND EMERGING TOPICS, 2011, 716 : 47 - 60
  • [8] Preventing Infection Post Hematopoietic Cell Transplantation
    Ghogomu, Jinwi T.
    Camero, Yesenia
    Friman, Karina
    US PHARMACIST, 2019, 44 (04) : 23 - 26
  • [9] Stem cell transplantation: Preventing relapse of transplant
    Craddock, Charles
    HEMASPHERE, 2018, 2 : 165 - 167
  • [10] MHC Class I Silencing Increases Graft Survival after Allogeneic Cell Transplantation
    Figueiredo, C.
    Wedekind, D.
    Mueller, T.
    Vahlsing, S.
    Horn, P. A.
    Seltsam, A.
    Blasczyk, R.
    TRANSPLANTATION, 2012, 94 (10) : 477 - 477