Separable enhancer sequences regulate the expression of the neural bHLH transcription factor neurogenin 1

被引：39

作者：

Nakada, Y ^{[1
]}

Parab, P ^{[1
]}

Simmons, A ^{[1
]}

Omer-Abdalla, A ^{[1
]}

Johnson, JE ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Ctr Basic Neurosci, Dallas, TX 75390 USA

来源：

DEVELOPMENTAL BIOLOGY | 2004年 / 271卷 / 02期

关键词：

transcriptional regulation; transgenic mice; dorsal-neural tube; ventral-neural tube; basic helix-loop-helix transcription factor; neurogenin; 1; spinal cord development;

D O I：

10.1016/j.ydbio.2004.04.021

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ngn1 is a basic helix-loop-helix (bHLH) transcription factor expressed in specific regions within the developing brain and spinal cord, sensory ganglia, and olfactory epithelium. We have identified sequences both 5' and 3' of the mouse ngn1 gene that function in regulating ngn1 expression, and each of these sequences contains distinct regulatory cassettes for different subregions of the expression domain. Enhancers for expression in ngn1 domains of the midbrain, hindbrain, trigeminal ganglia, and ventral-neural tube appear redundant and are spread both 5' and 3' of the ngn1 coding sequence. In contrast, a single discrete dorsal-neural tube enhancer was located in the 5' sequence that is conserved among mouse, human, chick, and zebrafish ngn1 genes. Functionally, this enhancer is both necessary and sufficient for driving expression of a heterologous reporter in transgenic mice specifically to the dorsal domain of ngn 1 expression in the spinal neural tube. Thus, sequences are identified that can be used to direct temporally and spatially restricted expression of heterologous genes to the developing neural tube. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：479 / 487

页数：9

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