Identification of a major blast resistance gene in the rice cultivar 'Tetep'

Analysis of near-isogenic lines (NILs) indicated the presence of a novel resistance gene in the indica rice cultivar 'Tetep' which was highly resistant to the rice blast fungus Magnaporthe grisea. 'Tetep' was crossed to the widely used susceptible cultivar 'CO39' to generate the mapping population. A Mendelian segregation ratio of 3 : 1 for resistant to susceptible F-2 plants further confirmed the presence of a major dominant locus, in 'Tetep', conferring resistance to the blast fungal isolate B157, corresponding to the international race IC9. Simple sequence length polymorphism (SSLP) was used for molecular genetic analysis. The analysis revealed that the SSLP marker RM 246 was linked to a novel blast resistance gene designated Pi-tp(t) in 'Tetep'.