Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice

被引：60

作者：

Uji, Yuya ^{[1
]}

Kashihara, Keita ^{[1
]}

Kiyama, Haruna ^{[1
]}

Mochizuki, Susumu ^{[1
]}

Akimitsu, Kazuya ^{[1
]}

Gomi, Kenji ^{[1
]}

机构：

[1] Kagawa Univ, Fac Agr, Plant Genome & Resource Res Ctr, Miki, Kagawa 7610795, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 12期

基金：

日本学术振兴会;

关键词：

MAP kinase; jasmonate; rice bacterial blight; salicylic acid; grain development; BACTERIAL-BLIGHT; GENE-EXPRESSION; EFFICIENT TRANSFORMATION; FUNCTIONAL-ANALYSIS; CONFERS RESISTANCE; DISEASE RESISTANCE; DEFENSE RESPONSES; UP-REGULATION; IDENTIFICATION; KINASE;

D O I：

10.3390/ijms20122917

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Jasmonic acid (JA) is a plant hormone that plays an important role in the defense response and stable growth of rice. In this study, we investigated the role of the JA-responsive valine-glutamine (VQ)-motif-containing protein OsVQ13 in JA signaling in rice. OsVQ13 was primarily located in the nucleus and cytoplasm. The transgenic rice plants overexpressing OsVQ13 exhibited a JA-hypersensitive phenotype and increased JA-induced resistance to Xanthomonas oryzae pv. oryzae (Xoo), which is the bacteria that causes rice bacterial blight, one of the most serious diseases in rice. Furthermore, we identified a mitogen-activated protein kinase, OsMPK6, as an OsVQ13-associating protein. The expression of genes regulated by OsWRKY45, an important WRKY-type transcription factor for Xoo resistance that is known to be regulated by OsMPK6, was upregulated in OsVQ13-overexpressing rice plants. The grain size of OsVQ13-overexpressing rice plants was also larger than that of the wild type. These results indicated that OsVQ13 positively regulated JA signaling by activating the OsMPK6-OsWRKY45 signaling pathway in rice.

引用

页数：16