A Novel System of Electrochemistry Coupled with Mass Spectrometry

被引：1
|
作者：
Cui Li-Li [1 ]
Wei Zhong-Lin [1 ]
Fei Qiang [1 ]
Li Ming [2 ]
机构：
[1] Jilin Univ, Coll Chem, Changchun 130012, Jilin, Peoples R China
[2] Natl Inst Metrol, Div Chem Metrol & Analyt Sci, Beijing 100029, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2019年 / 47卷 / 01期
关键词：
Electrochemistry coupled with mass spectrometry; Glutathione; Oxidation; Reduction; DISULFIDE BONDS; PROTEINS PRIOR; ELECTROSPRAY; IONIZATION; OXIDATION; ONLINE; PRINCIPLES; REDUCTION; CELL;
D O I：
10.19756/j.issn.0253-3820.181537
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel system of electrochemistry coupled with mass spectrometry ( EC-MS ) was developed. An electrospray ion source with a grounded needle design was used as the interface of the EC-MS, which avoided the damage of the electronic components of the potentiostat and significantly simplified instrument configuration. To evaluate the performance of the novel EC-MS system, the electrochemical reduction behavior of oxidized glutathione ( GSSG ) and the electrochemical oxidation behavior of reduced glutathione ( GSH ) were investigated. The experimental result showed that this system had high sensitivity, high reaction efficiency and high speed. As a new analytical approach, it has potential in identification of electrochemical reaction products and intermediates, proteomics and drug metabolism.
引用
收藏
页码：23 / 29
页数：7
相关论文
共 25 条
  • [1] Online electrochemistry/mass spectrometry in drug metabolism studies: principles and applications
    Baumann, Anne
    Karst, Uwe
    [J]. EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY, 2010, 6 (06) : 715 - 731
  • [2] Metabolic studies of tetrazepam based on electrochemical simulation in comparison to in vivo and in vitro methods
    Baumann, Anne
    Lohmann, Wiebke
    Schubert, Birthe
    Oberacher, Herbert
    Karst, Uwe
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (15) : 3192 - 3198
  • [3] Berkel G J V, 2002, ANAL CHEM, V74, P5047
  • [4] MECHANISM OF ELECTROSPRAY MASS-SPECTROMETRY - ELECTROSPRAY AS AN ELECTROLYSIS CELL
    BLADES, AT
    IKONOMOU, MG
    KEBARLE, P
    [J]. ANALYTICAL CHEMISTRY, 1991, 63 (19) : 2109 - 2114
  • [5] A setup for the coupling of a thin-layer electrochemical flow cell to electrospray mass spectrometry
    Bökman, CF
    Zettersten, C
    Sjöberg, PJR
    Nyholm, L
    [J]. ANALYTICAL CHEMISTRY, 2004, 76 (07) : 2017 - 2024
  • [6] USE OF A POROUS ELECTRODE FOR IN-SITU MASS SPECTROMETRIC DETERMINATION OF VOLATILE ELECTRODE REACTION PRODUCTS
    BRUCKENSTEIN, S
    RAOGADDE, R
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1971, 93 (03) : 793 - +
  • [7] An overview of electrochemistry combined with mass spectrometry
    Bruins, Andries P.
    [J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2015, 70 : 14 - 19
  • [8] Dual reductive/oxidative electrochemistry/liquid chromatography/mass spectrometry: Towards peptide and protein modification, separation and identification
    Bueter, Lars
    Frensemeier, Lisa M.
    Vogel, Martin
    Karst, Uwe
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2017, 1479 : 153 - 160
  • [9] Fundamental Principles and Practical Applications of Ambient Ionization Mass Spectrometry for Direct Analysis of Complex Samples
    Chen Huan-Wen
    Hu Bin
    Zhang Xie
    [J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2010, 38 (08) : 1069 - 1088
  • [10] An optimized electrochemistry-liquid chromatography-mass spectrometry method for studying guanosine oxidation
    Erb, Robert
    Plattner, Sabine
    Pitterl, Florian
    Brouwer, Hendrik-Jan
    Oberacher, Herbert
    [J]. ELECTROPHORESIS, 2012, 33 (04) : 614 - 621
123