The analysis of thyroxine in human serum by an 'exact matching' isotope dilution method with liquid chromatography/tandem mass spectrometry

被引：36
|
作者：
Hopley, CJ
Stokes, P
Webb, KS
Baynham, M
机构：
[1] Lab Govt Chemist, Teddington TW11 0LY, Middx, England
[2] Appl Biosyst Inc, Warrington WA3 7QH, Cheshire, England
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 10期
关键词：
D O I：
10.1002/rcm.1441
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the analysis of thyroxine in human serum, utilising 'exact matching' isotope dilution mass spectrometry (IDMS) in combination with liquid chromatography/tandem mass spectrometry (LC/MS/MS), has been developed with a limit of quantification of 0.5 ng g(-1) of thyroxine in human serum. The extraction and clean-up of thyroxine from serum involves an efficient protein-precipitation stage followed by a solid-phase extraction procedure to produce an extract essentially free from interfering compounds. The method is reproducible, with expanded uncertainties of less than 2%, and is relatively fast to perform. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码：1033 / 1038
页数：6
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