A Colloidal Gold Nanoparticle-Based Immunochromatographic Test Strip for Rapid and Convenient Detection of Staphylococcus Aureus

被引：28
|
作者：
Niu, Kaili [1 ,2 ]
Zheng, Xiaoping [3 ]
Huang, Chusen [1 ,2 ]
Xu, Kuan [1 ,2 ]
Zhi, Yuan [1 ,2 ]
Shen, Hebai [1 ,2 ]
Jia, Nengqin [1 ,2 ]
机构：
[1] Shanghai Normal Univ, Educ Minist Key Lab Resource Chem, Dept Chem, Shanghai 200234, Peoples R China
[2] Shanghai Normal Univ, Shanghai Key Lab Rare Earth Funct Mat, Dept Chem, Shanghai 200234, Peoples R China
[3] China Minist Agr, Food Qual Supervis & Inspect Ctr, Shanghai 200234, Peoples R China
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2014年 / 14卷 / 07期
关键词：
Staphylococcus Aureus; Gold Nanoparticles; Immunochromatography; Test Strip; POLYMERASE-CHAIN-REACTION; ESCHERICHIA-COLI; ASSAY; IDENTIFICATION; BACTERIA;
D O I：
10.1166/jnn.2014.8703
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 10(3) CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis.
引用
收藏
页码：5151 / 5156
页数：6
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